About MEAJO | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions to authors | Online submission | Subscribe | Advertise | Contact | Login 
Middle East African Journal of Ophthalmology Middle East African Journal of Ophthalmology
Users Online: 993   Home Print this page Email this page Small font sizeDefault font sizeIncrease font size

  Table of Contents 
Year : 2011  |  Volume : 18  |  Issue : 4  |  Page : 261-267  

Biopsy pathology in uveitis

Department of Ocular-Pathology and Uvea, Medical and Vision Research Foundation, Chennai, India

Date of Web Publication23-Nov-2011

Correspondence Address:
Jyotirmay Biswas
Department of Ocular-Pathology and Uvea, Medical and Vision Research Foundation, 18, College Road, Chennai - 600 006
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0974-9233.90126

Rights and Permissions

Uveitis is fraught with speculations and suppositions with regard to its etiology, progress and prognosis. In several clinical scenarios what may be perceived as due to a systemic infection may actually not be so and the underlying etiology may be an autoimmune process. Investigations in uveitis are sometimes the key in identification and management. Invasive techniques could be of immense value in narrowing down the etiology and help in identifying the cause. This article updates one on the invasive techniques used in biopsy such as anterior chamber paracentesis, vitreous tap and diagnostic vitrectomy, iris and ciliary body biopsy, choroidal and retinochoroidal biopsy and fine needle aspiration biopsy (FNAB). In populations where certain infections are endemic, the clinical scenario does not always respect a known presentation and the use of biopsy is resorted to as a sure way of confirming the etiology. Biopsies have a role in diagnosis of several inflammatory and infectious conditions in the eye and are pivotal in diagnosis in several dilemmas such as intraocular tumors and in inflammations. Appropriate and timely use of biopsy in uveitis could enhance the diagnosis and provide insight into the etiology, thus enabling precise management.

Keywords: Anterior Chamber Paracentesis, Choroidal and Retinochoroidal Biopsy and Fine Needle Aspiration Biopsy, Iris and Ciliary Body Biopsy, Vitreous Tap and Diagnostic Vitrectomy

How to cite this article:
Biswas J, Annamalai R, Krishnaraj V. Biopsy pathology in uveitis. Middle East Afr J Ophthalmol 2011;18:261-7

How to cite this URL:
Biswas J, Annamalai R, Krishnaraj V. Biopsy pathology in uveitis. Middle East Afr J Ophthalmol [serial online] 2011 [cited 2022 May 17];18:261-7. Available from: http://www.meajo.org/text.asp?2011/18/4/261/90126

   Introduction Top

Uveitis encompasses entities of varying duration, severity, location and above all a vast plethora of possible etiologies with quite similar and overlapping presentations.

Examination of a patient with uveitis needs to be meticulous as the incidence of association with systemic diseases is high. Uveitis is often diagnosed based on clinical features alone (e.g. Fuchs' heterochromic iridocyclitis, serpiginous choroiditis), with the help of ancillary tests (fundus fluorescein angiography, indocyanine green angiography, optical coherence tomography or with the help of laboratory tests (sarcoid, tuberculous and syphilitic uveitis). The importance and role of biopsy pathology is significant in masquerade syndromes, intraocular inflammation, infections, intraocular tumors and metastasis. With advancing surgical concepts and instrumentation, many of the biopsy procedures have become safe methods of reaching the correct diagnosis, thereby guiding appropriate treatment and also providing insights into the disease [Table 1].
Table 1: Various types of biopsy procedures used in uveitis

Click here to view

   Anterior Chamber Paracentesis Top

We describe herein a simple and safe technique, which can be performed in an outpatient department (OPD) setting, taking adequate aseptic precautions. The indication for an anterior chamber paracentesis may be many, but some of the common situations are in patients with masquerade syndromes [Figure 1], a hypopyon suspicious of infection [Figure 2], endophthalmitis and lens induced uveitis [Figure 3] and when performed can help in the diagnosis based on cytopathologic evidence [Figure 4].
Figure 1: Hypopyon in an eight year old girl

Click here to view
Figure 2: Anterior chamber aspirate showing multiple basophilic cells with pleomorphic nuclei suggestive of retinoblastoma (H and E, ×400)

Click here to view
Figure 3: Granulomatous uveitis with mutton fat keratic precipitates, posterior synechiae, traumatic cataract and ruptured anterior capsule of the lens

Click here to view
Figure 4: Anterior chamber aspirate of the same patient showing macrophages with engulfed matter (H and E, ×400)

Click here to view


After using local antibiotic drops and a local anesthetic, a tuberculin or 2cc syringe with a 27 to 30 gauge needle is used. However, if fibrin is present or in case of granulomatous uveitis, it is preferable to use a large bore of 25-26 gauge needle. The needle entry into the anterior chamber is oblique through the stroma via the lower limbus. This acts as a valvular self-sealing paracentesis wound on withdrawal of the needle. One should avoid touching the corneal endothelium and particularly the lens in phakic patients and should stay over the peripheral iris at all times. Obtain a 0.1 to 0.3-ml of aqueous, and on withdrawal external pressure is applied to the entrance with sterile cotton tipped applicator. A drop of antibiotic is instilled in the conjunctival sac and the eye is patched for half an hour after which the patient is re-examined to ensure anterior chamber reformation.

The procedure is quite safe. In case of infectious uveitis and endophthalmitis, a portion of the material should be sent to microbiology for direct smear for bacteria, fungus, acid fast bacilli, culture for bacteria, fungus and mycobacteria and polymerase chain reaction. Remaining fluid should undergo cytospin study to obtain better cell recovery for microscopic examination.

The authenticity of aqueous humor analysis has been reported following analysis of samples using smears, cultures, polymerase chain reaction (PCR) and real time PCR. Rothova et al., [1] have reported the usefulness of AC tap in isolation of infection in posterior uveitis. Further studies supporting this have also been reported by Rao et al,[2] on the utility of PCR analysis of aqueous humour samples in necrotizing retinitis. Cytopathologic features of aqueous aspirate [3] help in the diagnosis of lens induced uveitis, masquerade syndrome, delayed endophthalmitis, parasitic uveits to name a few [Table 2].
Table 2: Cytopathologic finding of aqueous aspirate in uveitis

Click here to view

Complications that can occur following AC tap are rare, nevertheless, one needs to be aware of the possibility of wound leak, hyphema, iritis and endophthalmitis.

PCR finds lot of application in ocular inflammation and uveitis and is now an important investigation. [4]

   Vitreous Tap and Diagnostic Vitrectomy Top

Material obtained from such procedures can undergo cytologic evaluation, PCR, detection of intraocular antibodies (by ELISA), flow cytometry and culture. [5],[6],[7],[8],[9] Vitreous biopsy can be vitreous tap or diagnostic vitrectomy. Such vitreous biopsy is required in infective posterior uveitis, recalcitrant posterior uveitis, suspected large cell lymphoma (reticulum cell sarcoma) particularly in the elderly population [Figure 5]. Obtaining vitreous samples by tap is easier and can be done in the outpatient department [10] however, material obtained is small. A diagnostic vitrectomy when performed provides large amount of material (though diluted), but needs to be done always in an operation theater. A cytologic analysis of vitreous aspirate can be of conclusive evidence in phacoanaphylactic uveitis,endophalmitis and in specific large cell lymphoma (reticulum cell sarcoma) [Table 3].
Table 3: Cytologic findings of vitreous biopsy

Click here to view
Figure 5: Color fundus montage photograph showing subretinal mass

Click here to view

Technique of vitreous tap

When performed in the out patient department, the technique is similar to that of anterior chamber paracentesis. As the perforation of the sclera is more painful than performing a keratocentesis, sub-conjunctival injection of 0.1 ml of 2% lidocaine can be given at the site of scleral perforation and entry into the vitreous space, 3.5-mm posterior to the limbus in phakic and 3.0 mm posterior to the limbus in aphakic/pseudophakic patients. It should be better done in the operation theater under special aseptic precautions.

The vitreous sampling is done using 25 to 23 gauge needles. Most eyes with long-standing intraocular inflammation have liquefied vitreous or fluid pockets within the vitreous. In such a situation, fine bored 25-guage needle can be used. When organization of vitreous is seen, 23-gauge needle is preferred. The needle is inserted in the vitreous cavity under direct visualization with slit lamp. The empty syringe withdraws the vitreous, and manipulating the stopcock, a similar quantity of antibiotic is injected into the vitreous cavity. After the injection the needle is slowly withdrawn from the eye. Complications with vitreous tap are vitreous hemorrhage, endophthalmitis, lens injury, retinal tears and secondary glaucoma.

Technique of vitreous sampling and biopsy

For analysis of the vitreous, it is essential to obtain undiluted vitreous specimen under sterile conditions intraoperatively. Newer techniques using vitrectomy with attachments and pneumovitrector have been described.

Doft et al., [11] obtained vitreous samples in eyes with endophthalmitis by directly connecting a syringe to the aspiration tube of the vitreous cutter. Others have used a collecting bottle with openings at both sides integrated into the aspiration system.

Smiddy et al., [12] obtained vitreous samples through a three-way stopcock through a manual aspiration.

Scholda and co-workers [13] have developed a new technique, in which a metal device is integrated into the aspiration system of the vitrectomy unit, which fits on standard laboratory plastic containers with integrated caps.

Peyman has described a full functional vitrectomy instrument (pneumovitrector) composed of an aspiration and cutting system combined with an infusion line for injecting air or gas into the vitreous cavity. [14]


A more thorough standard three-port vitrectomy can be performed, especially when therapeutically indicated in cases of endophthalmitis. The undiluted vitreous can be sampled and aspirated via a side-port.

All vitreous aspirated should be sent for examination as even the vitreous in the infusion line and vitrector can carry evidence of the pathology.

Tests and handling of aqueous and vitreous specimen

The specimen should be handled in such a way as to allow the maximum number of tests to be performed based on the clinical diagnosis. To maximize the chance of detecting the offending agents, the aqueous humor and vitreous specimen obtained should be divided in two equal volumes. One half of the specimen is used for the following tests


The samples should be immediately inoculated into blood agar, chocolate agar, brain-heart-infusion-broth (BHIB), thioglycolate fluid (maintained at body temperature), Sabouraud's agar,  Brucella More Details agar and BHIB with gentamicin (maintained at room temperature for fungal isolation).

Polymerase chain reaction

It is a powerful method to amplify specific sequences of DNA from a large complex mixture of DNA. For example, one can design PCR primers to amplify a single locus from an entire genome. PCR done on these samples particularly the vitreous sample can be a significant indicator of the presence of the organism.

A minimum volume of 0.05 ml should be reserved for this test especially when P. acnes, fungal endophthalmitis and uveitis of viral etiology are suspected. The other half of the original sample can be processed for the following tests.

Nested PCR means that two pairs of PCR primers were used for a single locus. The logic behind this strategy is that if the wrong locus were amplified by mistake, the probability is very low that it would also be amplified a second time by a second pair of primers.

Real time polymerase chain reaction

In molecular biology, real-time polymerase chain reaction, also called quantitative real time polymerase chain reaction (Q-PCR/qPCR/qrt-PCR) or kinetic polymerase chain reaction (KPCR), is a laboratory technique which is an evolution of PCR. In this technique, both evidence and quantification are done simultaneously and hence this investigation has the advantage of quantifying the load of the pathogen.


The entire sample can be spinned down, the supernatant transpipetted and the pellet resuspended in formalin or glutaraldehyde. These pellets are passed through two or more millipore filters and a number of specific staining methods including immunohistochemistry are carried out to identify the infiltrating cell types. [15] The other technique includes cytocentrifuge by cytospin method (about 1000 revolutions for 5 min). Cytopathologic findings of vitreous aspirate are given in [Table 3].


The supernatant obtained after spinning down the cellular components within the aqueous humor should be subjected to ELISA. The local production of specific antibodies within the ocular fluids is an important indication for the possible etiology especially when toxocara or toxoplasma is suspected. [16]

In Europe, the estimation of the Goldmann Witmer coefficient is done as given below:

Titer of antibody in aqueous concentration of serum globulin ×Titer of antibody in serum concentration of aqueous globulin.

= Goldmann Witmer coefficient

   Results Top

0.5 to 2 = No intraocular antibody production

2 to 4 = Suggestive of intraocular antibody production

> to 4 = Diagnostic of intraocular antibody production

Flow cytometric analysis

Flow cytometry (FCM) measures the physical and chemical properties of individual particles or cells moving in a single file in a fluid stream.

The most clearly defined application of FCM is in diagnostic surgical pathology. [17] It is an adjunct to histologic examination in the diagnosis of lymphoproliferative and leukemic processes. FCM is applied to the study of uveal melanomas, retinoblastomas and ocular lymphoid proliferations, especially masquerade syndrome. It is now also being used to provide valuable information regarding the ratio between the cytotoxic and helper T-cells, an indication for the immunologic events taking place during the course of the ocular disease.

In a recent study, Davis et al., [18] showed that in case of intraocular lymphoma positive predictive value (PPV) of cytology was 100%, but negative predictive value (NPV) was 60.9%. For infection, PPV for bacterial and fungal culture was 100% and NPV was 94.9%. CD 22 + ve B lymphocyte ≥ 20% of total cells on cytofluorographic analysis had a PPV of 88% for lymphoma.

   Iris and Ciliary Body Biopsy Top

Biopsy of iris and ciliary body is usually performed in suspected tumors in these regions. These lesions include glioneuromas, medulloepitheliomas, iridociliary cysts, leiomyomas, malignant melanomas and nematode granulomas. [19] Indications of iris and ciliary body biopsy in uveitic conditions are few and include the following:

  1. Metastatic lesions to the iris and ciliary body masquerading as uveitis.
  2. Ruptured iris cysts mimicking anterior uveitis.
  3. Iris and ciliary body nodules secondary to granulomatous conditions like tuberculosis and sarcoidosis. Moorthy and co-workers reported three patients with coccidioidomycosis iridocyclitis diagnosed by biopsy of iris nodules. [20]

   Choroidal and Retinochoroidal Biopsy Top

Lesions within the choroid can be difficult to differentiate clinically, although technological advances in non-invasive imaging have helped to monitor the size and the growth. The ophthalmologist has to consider the option of performing a choroidal or retinochoroidal biopsy. With advances in instrumentation and microsurgical techniques, endoretinal biopsy and chorioretinal biopsy can be performed easily and safely. Although the diagnosis of intraocular lymphoma can usually be made on the basis of diagnostic vitrectomy alone, sometimes it requires a more aggressive approach with choroidal biopsy, when pars plana vitrectomy and extensive medical examination fails to confirm the diagnosis.

Technique of retinal and choroidal biopsy

Trans-scleral choroidal biopsy

After conjunctival recession and hemostasis, the area of sclera overlying the lesion is marked. A half-thickness scleral trapdoor hinged posteriorly is created. 7-0 preplaced vicryl sutures are taken to allow rapid closure. Diathermy is applied to the circumference of the biopsy site. An opening of smaller dimension is made into the suprachoroidal space within the floor of the lamellar scleral dissection. This opening is enlarged to expose the choroid which is excised using Vannas scissors taking care to avoid perforation of the retina. The scleral trap door is then sutured. [21]

Internal approach for retinal, subretinal or chorioretinal biopsy

A vitreous biopsy is obtained at the beginning of the procedure before starting infusion. [22] The biopsy site in case of detached retina is selected at the junction of involved and normal retina. Unimanual bipolar endocautery is used at the margin of the proposed biopsy site. A biopsy specimen of at least 2 ×2 mm is then cut with 20 gauge vertical cutting scissors. The specimen is left attached at one corner, an intraocular forceps is used to remove it from the eye. After reattaching the retina, endolaser is applied to the biopsy site and long-acting gas or silicone oil tamponade is used. In case of attached retina, combined retinal and choroidal biopsy is obtained. Intense endo-diode laser is first applied around the margin of the biopsy site. Two minutes later a 2×2 mm biopsy specimen is cut within the margins of the laser burns with vertically cutting intraocular scissors until sclera is visible.

   Fine Needle Aspiration Biopsy Top

Biopsy specimens are performed when the cause of a disease is uncertain or its extent or exact character is in doubt. Aspiration biopsy had been first described as early as 1847, though the first report was published in 1930. [23] Jacobiec et al., proposed fine needle aspiration biopsy (FNAB) for intraocular tumors in 1979. FNAB offers a histopathologic correlation to the clinical diagnosis in the case of atypical presentation of intraocular tumors. It aids in effective planning and management; it enables histopathological diagnosis without sacrificing the eye or having to resort to open biopsy methods

FNAB has been recommended in,

  1. Cases of suspected infectious subretinal lesions (abscesses or tuberculomas) mimicking as choroidal tumors. Gregor and coworkers diagnosed a Nocardia asteroides subretinal abscess following a trans-vitreal FNAB. [24]
  2. Cases where diagnosis is difficult, distinction between benign and malignant lesion is not clear, all ancillary tests are inconclusive and where therapeutic decisions will be made on the basis of cytological findings.

  1. Patients with metastatic disease of the choroid but with no primary.
  2. Cases where patient refuses recommended therapy until histopathological confirmation is obtained.

Techniques of fine needle aspiration biopsy


  1. Limbal route is used to approach anterior uveal lesions [25] eg. iris lesions, or in aphakic patients for posterior ciliary body lesions.
  2. In the posterior segment lesions, [26] the possible approaches are

Pars plana trans-vitreal approach

In this approach, the needle is passed from the pars plana region (3.5 mm from the limbus) in the quadrant opposite to the lesion, through the vitreous gel. For some of the eyes with tumors located posteriorly, a vitrectomy needs to be performed before aspiration.

Corneo-limbal approach

This approach through the zonules prevents dissemination of the tumor mass through the needle track. This approach is used in patients with retinoblastoma, a highly friable tumor, as the chance of needle track dissemination is extremely high. Through a corneolimbal approach the needle passes through multiple planes, thus wiping out the tumor cells as the needle is removed from the eye.

Sub retinal fine needle aspiration biopsy

This can be done in cases of sub retinal abscesses and tuberculomas, considering the site is approachable.


  1. The most common complication of FNAB is bleeding from the site of the needle track.
  2. Orbital dissemination of tumor cells and distant metastatic spread caused by tumor implantation along the needle track have been reported which are rare now.
  3. Iatrogenic retinal perforations are unavoidable by the indirect needle approach to the choroidal lesions and can theoretically cause a retinal detachment after FNAB.

Biopsy of intraocular lymphoma

Steroids should be stopped before diagnostic procedures to increase the diagnostic yield in suspected lymphoma cases. Samples should be obtained from the densest part of the infiltrate. [27] Also, it is worthy to note that lymphoma cells undergo apoptosis 90-100μ from their blood supply. If a distinct sub retinal mass is present, a direct sub retinal biopsy is better than a vitreous tap. The cells of lymphoma are fragile [Figure 6]. Due to the associated presence of inflammatory cytokines, they may degenerate and also the DNA degrades resulting in false negative results. [28] Samples may be placed in cell culture media to improve viability of cells. Interleukin 10 (IL- 10) levels have been found to be elevated in serum and vitreous of patients with non Hodgkins lymphoma while interleukin 6 (IL- 6) levels are elevated in the vitreous of patients with intraocular inflammation. Whitcup et al., found in a study that IL 10 levels exceeded the IL 6 levels in all 5 of study patients with primary central nervous system lymphoma involving the eye but in none of 13 patients with uveitis. [29]
Figure 6: Fine needle aspiration biopsy showing pleomorphic malignant cells in necrotic background (H and E, ×100)

Click here to view

Biopsy in vasculitis

Biopsy of the temporal arteries is often performed for suspected inflammatory bowel disease (Crohn's disease and ulcerative colitis) which can present with uveitis both anterior non granulomatous or posterior type. Frequent biopsies are taken to assess the activity of disease and to assess changes that precede malignancy. The FNAB has a sensitivity and specificity rate of 84 and 98% respectively. [30]

   Conclusion Top

In the future, newer association with infection, systemic disease and genetic patterns in uveitis will arise and needs to be explored further, focusing on the pathogenesis of the underlying ocular inflammation. However, while translating these research findings into clinical application, several points like cost effectiveness will have to be considered. The different immunological hypotheses of etiology of uveitis needs to be verified, and adopted or rejected and management done accordingly. Biopsy pathology in ocular inflammation has no doubt a well defined place in the investigation protocol especially considering the rarity of certain types and the overlapping features that may co exist in the same patient. The most important consideration is that biopsy proven pathogenesis and etiology is reliable and treatment can be instituted based on the histopathological outcome.

   References Top

1.Van Der Lelij A, Rothova A. Diagnostic anterior chamber paracentesis in uveitis: A safe procedure. Br J Ophthalmol 1997;81:976-9.  Back to cited text no. 1
2.Tran TH, Rozenberg F, Cassoux N, Rao NA, Le Hoang P, Bodaghi B. Polymerase chain reaction analysis of aqueous humour samples in necrotising retinitis. Br J Ophthalmol 2003;87:79-83.  Back to cited text no. 2
3.Biswas J. Pathology of uveitis. Afro-Asian J Ophthalmol 1995;11:205-8.  Back to cited text no. 3
4.Arif Mulla MD, Biswas J,Madhavan HN ,Therese L. The role of polymerase reaction in the diagnosis of infectious uveitis. Abstract accepted for publication in Indian Journal of Ophthalmology.www.aios.org/proceed 10/UVEA/Uvea 6.pdf.  Back to cited text no. 4
5.Priem H, Verbraeken H, de Laey JJ. Diagnostic problems in chronic vitreous inflammation. Graefes Arch Clin Exp Ophthalmol 1993;231:453-6.  Back to cited text no. 5
6.Palexas GN, Green WR, Goldberg MF, Ding Y. Diagnostic parsplana vitrectomy report of a 21-year retrospective study. Trans Am Ophthalmol Soc 1995;93:281-308.  Back to cited text no. 6
7.Verbraeken H. Diagnostic vitrectomy and chronic uveitis. Grafes Arch Clin Exp Ophthalmol 1996;234 Suppl:S2-7.  Back to cited text no. 7
8.Liu K, Klintworth GK, Dodd LG. Cytologic findings in vitreous fluids: Analysis of 74 specimens. Acta Cytol 1999;43:201-6.  Back to cited text no. 8
9.Coupland SE, Bechrakis NE, Anastassiou G, Foerster AM, Heiligenhaus A, Pleyer U, et al. Evaluation of vitrectomy specimens and chorioretinal biopsies in the diagnosis of primary intraocular lymphoma in patients with Masquerade syndrome. Graefes Arch Clin Exp Ophthalmol 2003;241:860-70.  Back to cited text no. 9
10.Lobo A, Lightman S. Vitreous aspiration needle tap in the diagnosis of intraocular inflammation. Ophthalmology 2003;110:595-9.  Back to cited text no. 10
11.Doft BK, Donelly K. A single sclerotomy vitreous biopsy in endophthalmitis. Arch Ophthalmol 1991;109:465.  Back to cited text no. 11
12.Smiddy WE, Michels RG, de Bustros, de la Cruz Z, Green WR. Histopathology of tissue removed during vitrectomy for impending macular holes. Am J Ophthalmol 1989;108:360-4.  Back to cited text no. 12
13.Scholda CD, Egger SF, Lakits A, Haddad R. A system for obtaining undiluted intraoperative vitreous biopsy specimen. Arch Ophthalmol 1996;114:1271-2.  Back to cited text no. 13
14.Peyman GA. A Pneumovitrector for the diagnostic biopsy of the vitreous. Ophthalmol Surg Laser 1996;27:246-7.  Back to cited text no. 14
15.Ben Erza D. Diagnostic intraocular interventions. In: Ben Erza D, editor. Ocular inflammation: Basic and clinical concepts. Martin Dunitz Ltd.; 1999. p. 83-9.  Back to cited text no. 15
16.Joynson DH, Payne RA, Rawal BK. Potential role of IgG avidity for diagnosing toxoplasmosis. J Clin Pathol 1990;43:1032-3.  Back to cited text no. 16
17.Crofty TB, Campbell RJ. Flow cytometry. Ophthalmol Clin North Am 1995;8:37-46.  Back to cited text no. 17
18.Davis JL. Intraocular lymphoma. Ocul Immunol Inflamm 2004;12:7-16.  Back to cited text no. 18
19.Johnston RL, Tufail A, Lightman S, Luthert PJ, Pavesio CE, Cooling RJ, et al. Retinal and choroidal biopsies are helpful in unclear uveitis of suspected infectious or malignant origin. Ophthalmology 2004;111:522-8.  Back to cited text no. 19
20.Moorthy RS, Rao NA, Sidikaro Y, Foos RY. Coccidioidomycosis iridocyclitis. Ophthalmology 1994;101:1923-8.  Back to cited text no. 20
21.Foulds WS. The uses and limitations of intraocular biopsy. Eye (Lond) 1992;6:11-27.  Back to cited text no. 21
22.Rutzen AR, Ortega-Larrocea G, Dugel PU, Chong LP, Lopez PF, Rao NA. Retinal and choroidal biopsy in intraocular inflammation: A clinic-pathologic study. Trans Am Ophthalmol Soc 1994;92:431-58.  Back to cited text no. 22
23.Midnea E, Segato T, Piermarocchi S, Boccato P. Fine needle aspiration biopsy in ophthalmology. Surv Ophthalmol 1985;29:410-22.  Back to cited text no. 23
24.Gregor RJ, Chong CA, Augsburger JJ, Eagle RC Jr, Carlson KM, Jessup M, et al. Endogenous Nocardia asteroides subretinal abscess diagnosed by transvitreal fine needle aspiration biopsy. Retina 1989;9:118-21.  Back to cited text no. 24
25.Shanmugam MP, Biswas J. Fine needle aspiration biopsy in the diagnosis of intraocular lesions. Indian J Ophthalmol 1997;45:105-8.  Back to cited text no. 25
[PUBMED]  Medknow Journal  
26.Glasgow BJ, Straatsma BR, Kreiger AE. Fine-needle aspiration of the posterior segment intraocular tumors. Ophthalmol Clin North Am 1995;8:59-72.  Back to cited text no. 26
27.Zaldivar RA, Martin FD, Holden JT, Grossniklaus HE. Primary intraocular lymphoma: Clinical, cytologic, and flow cytometric analysis. Ophthalmology 20004;111:1762-7.  Back to cited text no. 27
28.Murray PI, Hoekzema R, van Haren MA, de Hon FD, Kijlstra A. Aqueous humor interleukin-6 levels in uveitis. Invest Ophthalmol Vis Sci 1990;31:917-20.  Back to cited text no. 28
29.Whitcup SM, Stark-Vancs V, Soloman D, Podgor MJ, Nussenblatt RB, Chan CC. Association of Interleukin 10 in the vitreous and cerebrospinal fluid and primary central nervous system lymphoma. Arch Ophthalmol 1997;115:1157-60.   Back to cited text no. 29
30.Midnea E, Segato T, Piermarocchi S, Boccato P. Fine needle aspiration biopsy in ophthalmology. Surv Ophthalmol 1985;29:410-22.  Back to cited text no. 30


  [Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6]

  [Table 1], [Table 2], [Table 3]

This article has been cited by
1 Intraocular biopsy in uveitis
Gazal Patnaik, Radha Annamalai, Jyotirmay Biswas
Indian Journal of Ophthalmology. 2020; 68(9): 1838
[Pubmed] | [DOI]
2 Meet the challenge
Natarajan, S.
Indian Journal of Ophthalmology. 2013; 61(6): 251-252
3 Rosai-Dorfman disease presenting as choroidal melanoma: A case report and review of the literature
Vermeulen, T.L. and Isaacs, T.W. and Spagnolo, D. and Amanuel, B.
Graefećs Archive for Clinical and Experimental Ophthalmology. 2013; 251(1): 295-299
4 Vitreous Diagnosis in Neoplastic Diseases
Mónica Asencio-Duran,José Luis Vallejo-Garcia,Natalia Pastora-Salvador,Agustín Fonseca-Sandomingo,Mario R. Romano
Mediators of Inflammation. 2012; 2012: 1
[Pubmed] | [DOI]
5 Vitreous diagnosis in neoplastic diseases
Asencio-Duran, M. and Vallejo-Garcia, J.L. and Pastora-Salvador, N. and Fonseca-Sandomingo, A. and Romano, M.R.
Mediators of Inflammation. 2012; 2012(930704)


    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Access Statistics
    Email Alert *
    Add to My List *
* Registration required (free)  

  In this article
    Anterior Chamber...
    Vitreous Tap and...
    Iris and Ciliary...
    Choroidal and Re...
    Fine Needle Aspi...
    Article Figures
    Article Tables

 Article Access Statistics
    PDF Downloaded265    
    Comments [Add]    
    Cited by others 5    

Recommend this journal