|Year : 2015 | Volume
| Issue : 3 | Page : 383-385
Postkeratoplasty keratitis caused by Abiotrophia defectiva: An unusual cause of graft infection
Guru Prasad Manderwad1, Somasheila I Murthy2, Swapna Reddy Motukupally1
1 Jhaveri Microbiology Center, Professor Brien Holden Eye Research Center, Hyderabad, Telangana, India
2 L. V. Prasad Eye Institute, Kallam Anji Reddy Campus, Banjara Hils, Hyderabad, Telangana, India
|Date of Web Publication||1-Jul-2015|
Somasheila I Murthy
Cornea and Anterior segment Services, L. V. Prasad Eye Institute, Hyderabad - 500 034, Telangana
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Abiotrophia defectiva is a nutritional variant of Streptococci. We describe a case of microbial keratitis due to A. defectiva in a patient who had undergone penetrating keratoplasty and was on corticosteroid therapy for recent graft rejection. Isolation of this organism confirmed this to be an opportunistic infection.
Keywords: Abiotrophia defectiva, Keratitis, VITEK-2 Compact System
|How to cite this article:|
Manderwad GP, Murthy SI, Motukupally SR. Postkeratoplasty keratitis caused by Abiotrophia defectiva: An unusual cause of graft infection. Middle East Afr J Ophthalmol 2015;22:383-5
|How to cite this URL:|
Manderwad GP, Murthy SI, Motukupally SR. Postkeratoplasty keratitis caused by Abiotrophia defectiva: An unusual cause of graft infection. Middle East Afr J Ophthalmol [serial online] 2015 [cited 2022 Jan 28];22:383-5. Available from: http://www.meajo.org/text.asp?2015/22/3/383/150631
| Introduction|| |
To report Abiotrophia defectiva as the etiological agent in the case of graft infection isolated using the VITEK-2 (BioMerieux SA, France) compact system.
| Case Report|| |
A 77-year-old patient presented for a follow-up for his ongoing ocular condition. Past ocular history was significant for a known diagnosis of bilateral lattice stromal corneal dystrophy, left eye more severe than the right eye, with his first visit to our center 10 years ago. There was positive family history of a similar condition in his mother and younger brother. He had undergone multiple surgeries: Phototherapeutic keratectomy, followed by cataract surgery with intraocular lens implantation in the right eye 9 years ago and a penetrating keratoplasty combined with cataract surgery and posterior chamber intraocular lens implantation in the left eye 8 years ago. He was on regular follow-up and had a best-corrected visual acuity of 20/60 in the right eye (with residual lattice dystrophic opacities) and 20/20 in the left eye until 6 months prior to his last presentation. A month prior presentation, the patient presented with a significant reduction in vision and was diagnosed with acute allograft rejection, which was treated with intensive corticosteroid therapy. However, the graft failed to recover.
At the time of presentation, the patient complained of sudden pain and redness lasting 1-week. The patient was still using prednisolone acetate 1% eye drops once a day. Best-corrected visual acuity was 20/80 in the right eye and counting fingers at 2 m in the left eye. Examination of his left eye indicated the corneal graft was in place, with an oval, well-defined epithelial defect measuring 3 mm in diameter with an underlying stromal infiltrate and 25% thinning, located at the periphery of the graft at the 9'o clock position. The surrounding cornea showed grade 3 stromal edema. Corneal Scrapping were performed for laboratory assessment (see below). Based on microbiology results of the corneal scrapings, treatment was initiated with fortified topical cefazolin 1% and topical ciprofloxacin 0.3% every hour, along with topical atropine sulfate 1% 3 times a day. At follow-up 3 weeks later, the infiltrate resolved with scarring. Final visual outcome was 20/400, the graft remained edematous due to the secondary graft failure following allograft rejection [Figure 1].
|Figure 1: Slit lamp photograph of the left eye showing area of corneal scar at the graft-host junction (arrow) corresponding to the resolved infiltrate|
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After instilling 0.5% topical proparacaine, corneal scrapping were collected using a number 15 sterile blade. Corneal scrapings were processed based on our institute's microbiology protocol. The scrapping were first transferred on slides for 10% potassium hydroxide (KOH) with calcoflour white white (CFW) preparation, gram and giemsa staining as well as inoculation in the following enriched media: Chocolate agar, blood agar, broth including brain heart infusion broth, thioglycolate broth and for fungal isolation media including, Sabroud's dextrose agar and potato dextrose agar. Gram's stain showed the presence of polymorphs 0-2/oil immersion field (OIF), epithelial cells 0-2/OIF and Gram-positive cocci (GPC) in pairs and chains 0-plenty/OIF [Figure 2]a. Giemsa stain showed the presence of polymorphs 0-2/OIF, cocci in pairs and chains 0-plenty/OIF. KOH + CFW was negative. Small translucent colonies were noted on blood agar with β-hemolysis after 24 h [Figure 2]b. Culture smear revealed GPC in chains. The organism was catalase negative and optochin resistant. For the identification of the organism, the culture was subjected to the VITEK-2 compact system (BioMerieux SA, France). The organism was identified as A. defectiva with a record of excellent identification with 99.9% probability. Antibiotic drug sensitivity using Kirby-Baeur disc diffusion showed sensitivity to amikacin, cefazolin, ofloxacin, cefuroxime, gentamicin, vancomycin, gatifloxacin, moxifloxacin, ciprofloxacin and chloramphenicol.
|Figure 2: (a) Direct smear shows the presence of Gram-positive cocci in corneal scrapping. (b) Translucent colonies were noted on blood agar with β-haemolysis|
Click here to view
| Discussion|| |
Abiotrophia (which means "life nutrition deficiency") is the nutritional variant of Streptococci. It is part of the normal flora of the oral cavity, the urogenital and intestinal tracts, but is not normally found in the conjunctival flora.  In humans, it has been reported to cause serious infections, including endocarditis,  brain abscesses,  septic arthritis,  and bacteremia. 
This organism has rarely been implicated as a cause of ocular infections and has been previously reported in cases of endophthalmitis and keratitis. ,, Keay et al. were the first to report the association of this organism with infiltrative keratitis associated with extended wear with hydrogel lenses.  Rudolph et al. described Abitrophia in keratitis cases.  A study from France reported A. defectiva as the cause of infectious crystalline keratopathy.  In most cases, the infection responded to vancomycin, and the final visual outcome was reasonably good.  With improvement in microbiological techniques including the application of the VITEK-2 Compact system, rare organisms, which may have been missed earlier can now be identified. The VITEK-2 compact system is used for identifying the organisms. It is an automated platform, and the identification is rapid and accurate. This system detects growth of the bacteria based on the metabolic changes and is based on fluorescence technology. It accurately identifies the organisms up to the species level and a good level discrimination between species. ,
The current report shows that the clinical presentation of A. defectiva was similar to graft infections due to other GPC, such as Streptococcus or Staphylococcus sp. with hallmark features of well-circumscribed infiltrate and intense inflammatory reaction. It was noted that the organism was sensitive to most of the commonly used antibiotics and was associated with a good outcome in the present case. It is possible that this organism has been under-reported as it may not be identified by other techniques and may have been grouped with other GPC-related infections. The clinical presentation does not help in differentiating this entity. The use of enriched media for culture and isolation, application of VITEK-2 compact system (BioMerieux SA, France), lead to the detection of rare, fastidious organism.
| Acknowledgments|| |
Hima Bindu Surisetti and Asadullah Syed for the technical help Hyderabad Eye Research Foundation and Hyderabad Eye Institute.
| References|| |
Ruoff KL. Nutritionally variant Streptococci. Clin Microbiol Rev 1991;4:184-90.
Wijetunga M, Bello E, Schatz I. Abiotrophia
endocarditis: A case report and review of literature. Hawaii Med J 2002;61:10-2.
Zenone T, Durand DV. Brain abscesses caused by Abiotrophia defectiva
: Complication of immunosuppressive therapy in a patient with connective-tissue disease. Scand J Infect Dis 2004;36:497-9.
Taylor CE, Fang MA. Septic arthritis caused by Abiotrophia defectiva
. Arthritis Rheum 2006;55:976-7.
Lopardo H, Mastroianni A, Casimir L. Bacteremia due to Abiotrophia defectiva
in a febrile neutropenic pediatric patient. Rev Argent Microbiol 2007;39:93-4.
Namdari H, Kintner K, Jackson BA, Namdari S, Hughes JL, Peairs RR, et al. Abiotrophia
species as a cause of endophthalmitis following cataract extraction. J Clin Microbiol 1999;37:1564-6.
Horstkotte MA, Dobinsky S, Rohde H, Knobloch JK, Hassenstein A, Kalitzky M, et al. Abiotrophia defectiva
endophthalmitis with retinal involvement and infiltrative keratitis: Case report and review of the literature. Eur J Clin Microbiol Infect Dis 2010;29:727-31.
Esteban J, Montero-Sánchez R, Ortiz A, Yáñez F. Postoperative endophthalmitis due to Abiotrophia defectiva
. Enferm Infecc Microbiol Clin 2005;23:455.
Keay L, Harmis N, Corrigan K, Sweeney D, Willcox M. Infiltrative keratitis associated with extended wear of hydrogel lenses and Abiotrophia defectiva
. Cornea 2000;19:864-9.
Rudolph T, Welinder-Olsson C, Lind-Brandberg L, Stenevi U 16S rDNA PCR analysis of infectious keratitis: A case series. Acta Ophthalmol Scand 2004;82:463-7.
Abry F, Sauer A, Riegel P, Saleh M, Gaucher D, Speeg-Schatz C, et al.
Infectious crystalline keratopathy caused by Streptococcus Abiotrophia defectiva
. Cornea 2010;29:934-6.
Spanu T, Sanguinetti M, Ciccaglione D, D'Inzeo T, Romano L, Leone F, et al.
Use of the VITEK-2 system for rapid identification of clinical isolates of Staphylococci from bloodstream infections. J Clin Microbiol 2003;41:4259-63.
Funke G, Monnet D, deBernardis C, von Graevenitz A, Freney J. Evaluation of the VITEK-2 system for rapid identification of medically relevant gram-negative rods. J Clin Microbiol 1998;36:1948-52.
[Figure 1], [Figure 2]
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